Open AccessCrystallization and preliminary X‐ray diffraction studies of ferredoxin reductase from Leptospira interrogans
Author(s) -
Ferrarezi Thiago,
Nascimento Alessandro S.,
Ceccarelli Eduardo A.,
CatalanoDupuy Daniela L.,
Polikarpov Igor
Publication year - 2006
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309106020136
Subject(s) - ferredoxin , crystallization , leptospira interrogans , x ray , crystallography , materials science , chemistry , leptospira , physics , optics , microbiology and biotechnology , biology , biochemistry , enzyme , organic chemistry , serotype
Ferredoxin‐NADP + reductase (FNR) is an FAD‐containing enzyme that catalyzes electron transfer between NADP(H) and ferredoxin. Here, results are reported of the recombinant expression, purification and crystallization of FNR from Leptospira interrogans , a parasitic bacterium of animals and humans. The L. interrogans FNR crystals belong to a primitive monoclinic space group and diffract to 2.4 Å resolution at a synchrotron source.