Open AccessCrystallization and preliminary X‐ray diffraction analysis of a tRNA Ser acceptor‐stem microhelix
Author(s) -
Förster Charlotte,
Krauss Norbert,
Brauer Arnd B. E.,
Szkaradkiewicz Karol,
Brode Svenja,
Hennig Klaus,
Fürste Jens P.,
Perbandt Markus,
Betzel Christian,
Erdmann Volker A.
Publication year - 2006
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309106016472
Subject(s) - crystallization , transfer rna , x ray crystallography , crystallography , diffraction , acceptor , x ray , materials science , chemistry , physics , rna , biochemistry , optics , gene , organic chemistry , condensed matter physics
In order to understand elongator tRNA Ser and suppressor tRNA Sec identity elements, the respective acceptor‐stem helices have been synthesized and crystallized in order to analyse and compare their structures in detail at high resolution. The synthesis, crystallization and preliminary X‐ray diffraction results for a seven‐base‐pair tRNA Ser acceptor‐stem helix are presented here. Diffraction data were collected to 1.8 Å, applying synchrotron radiation and cryogenic cooling. The crystals belong to the monoclinic space group C 2, with unit‐cell parameters a = 36.14, b = 38.96, c = 30.81 Å, β = 110.69°.