Open AccessCrystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding
Author(s) -
SanchezWeatherby Juan,
Southall Stacey,
Oubrie Arthur
Publication year - 2006
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309106014862
Subject(s) - streptomyces coelicolor , maltose , escherichia coli , dehydrogenase , chemistry , biochemistry , monosaccharide , crystallization , enzyme , organic chemistry , gene , mutant
The soluble quinoprotein glucose dehydrogenase oxidizes glucose, maltose and a variety of other monosaccharides and disaccharides to the corresponding lactones. An efficient microseeding protocol is reported to produce crystals of three variants that display reduced activity towards maltose. Similar cross‐seeding protocols to grow crystals of homologues from Escherichia coli and Streptomyces coelicolor are described.