Open AccessPreliminary X‐ray investigations of several crystal forms of the ferripyoverdine FpvA outer membrane receptor from Pseudomonas aeruginosa bound to ferripyoverdine
Author(s) -
Wirth Christophe,
Hoegy Françoise,
Pattus Franc,
Cobessi David
Publication year - 2006
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309106012553
Subject(s) - bacterial outer membrane , pseudomonas aeruginosa , crystallization , membrane , chemistry , biophysics , crystal (programming language) , crystallography , inner membrane , domain (mathematical analysis) , biochemistry , biology , bacteria , organic chemistry , escherichia coli , mathematics , genetics , computer science , programming language , mathematical analysis , gene
Ferripyoverdine transport across the outer membrane of Pseudomonas aeruginosa by the pyoverdine receptor FpvA and the transcriptional regulation of FpvA involve interactions of the FpvA N‐terminal TonB box and signalling domain with proteins from the inner membrane. Several crystallization conditions of FpvA–Pvd‐Fe solubilized in C 8 E 4 detergent were obtained and X‐ray data were collected from three crystal forms. The resolution limits range from 3.15 to 2.7 Å depending on the crystal form. From preliminary analysis of the electron‐density maps, the first full‐length structure of an outer membrane receptor including a signalling domain should be determined.