Open AccessCrystallization and preliminary X‐ray diffraction analysis of Nsp15 from SARS coronavirus
Author(s) -
Ricagno Stéfano,
Coutard Bruno,
Grisel Sacha,
Brémond Nicolas,
Dalle Karen,
Tocque Fabienne,
Campanacci Valérie,
Lichière Julie,
Lantez Violaine,
Debarnot Claire,
Cambillau Christian,
Canard Bruno,
Egloff MariePierre
Publication year - 2006
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309106009407
Subject(s) - endoribonuclease , escherichia coli , coronavirus , recombinant dna , microbiology and biotechnology , chemistry , transcription (linguistics) , rna , enzyme , biology , virology , gene , crystallography , biochemistry , rnase p , medicine , covid-19 , linguistics , philosophy , disease , pathology , infectious disease (medical specialty)
The non‐structural protein Nsp15 from the aetiological agent of SARS (severe acute respiratory syndrome) has recently been characterized as a uridine‐specific endoribonuclease. This enzyme plays an essential role in viral replication and transcription since a mutation in the related H229E human coronavirus nsp15 gene can abolish viral RNA synthesis. SARS full‐length Nsp15 (346 amino acids) has been cloned and expressed in Escherichia coli with an N‐terminal hexahistidine tag and has been purified to homogeneity. The protein was subsequently crystallized using PEG 8000 or 10 000 as precipitants. Small cubic crystals of the apoenzyme were obtained from 100 nl nanodrops. They belong to space group P 4 1 32 or P 4 3 32, with unit‐cell parameters a = b = c = 166.8 Å. Diffraction data were collected to a maximum resolution of 2.7 Å.