Open AccessStructure of the manganese superoxide dismutase from Deinococcus radiodurans in two crystal forms
Author(s) -
Moe Elin,
Dennis Rebecca J.,
Micossi Elena,
McCarthy Joanne,
Gordon Elspeth J.,
KozielskiStuhrmann Sigrid,
Leonard Gordon A.,
McSweeney Sean
Publication year - 2006
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309106008402
Subject(s) - deinococcus radiodurans , manganese , isostructural , monoclinic crystal system , crystallography , chemistry , superoxide dismutase , crystal structure , crystal (programming language) , escherichia coli , molecular replacement , deinococcus , resolution (logic) , enzyme , biochemistry , dna , organic chemistry , computer science , programming language , gene , artificial intelligence
The structure of the manganese superoxide dismutase (Mn‐SOD; DR1279) from Deinococcus radiodurans has been determined in two different crystal forms. Both crystal forms are monoclinic with space group P 2 1 . Form I has unit‐cell parameters a = 44.28, b = 83.21, c = 59.52 Å, β = 110.18° and contains a homodimer in the asymmetric unit, with structure refinement ( R = 16.8%, R free = 23.6%) carried out using data to d min = 2.2 Å. Form II has unit‐cell parameters a = 43.57, b = 87.10, c = 116.42 Å, β = 92.1° and an asymmetric unit containing two Mn‐SOD homodimers; structure refinement was effected to a resolution of 2.0 Å ( R = 17.2%, R free = 22.3%). The resulting structures are compared with that of Mn‐SOD from Escherichia coli , with which they are shown to be essentially isostructural.