Open AccessCrystallization and preliminary X‐ray crystallographic analysis of a galactose‐specific lectin from Dolichos lablab
Author(s) -
Lavanya Latha V.,
Kulkarni Kiran A.,
Nagender Rao R.,
Siva Kumar N.,
Suguna K.
Publication year - 2006
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309106001448
Subject(s) - dolichos , lectin , crystallization , tetramer , galactose , crystallography , resolution (logic) , materials science , chemistry , biology , biochemistry , botany , organic chemistry , artificial intelligence , computer science , enzyme
The galactose‐specific lectin from the seeds of Dolichos lablab has been crystallized using the hanging‐drop vapour‐diffusion technique. The crystals belong to space group P 1, with unit‐cell parameters a = 73.99, b = 84.13, c = 93.15 Å, α = 89.92, β = 76.01, γ = 76.99°. X‐ray diffraction data to a resolution of 3.0 Å have been collected under cryoconditions (100 K) using a MAR imaging‐plate detector system mounted on a rotating‐anode X‐ray generator. Molecular‐replacement calculations carried out using the available structures of legume lectins as search models revealed that the galactose‐specific lectin from D. lablab forms a tetramer similar to soybean agglutinin; two such tetramers are present in the asymmetric unit.