Open AccessCrystallization and preliminary crystallographic study of the yeast Malassezia sympodialis allergen Mala s 1
Author(s) -
Vilhelmsson Monica,
Hallberg B. Martin,
Rasool Omid,
Zargari Arezou,
Scheynius Annika,
Achour Adnane
Publication year - 2006
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309105041011
Subject(s) - yeast , allergen , chemistry , recombinant dna , escherichia coli , microbiology and biotechnology , food science , biology , immunology , biochemistry , allergy , gene
The opportunistic yeast Malassezia sympodialis can act as an allergen and elicit specific IgE‐ and T‐cell reactivity in patients with atopic eczema. The first identified major allergen from M. sympodialis , Mala s 1, is present on the cell surface of the yeast. Recombinant Mala s 1 was expressed in Escherichia coli , purified and refolded in a soluble form. Crystals of Mala s 1 were obtained in 25% PEG 8K, 0.2 M (NH 4 ) 2 SO 4 . Crystals belong to space group P 2 1 2 1 2, with unit‐cell parameters a = 44.4, b = 163.7, c = 50.6 Å, and diffract to 1.35 Å resolution.