Open AccessPurification, crystallization and preliminary X‐ray crystallographic analysis of rice lectin from Oryza sativa
Author(s) -
Huang YenChieh,
Lin YiHung,
Shih ChiaHao,
Shih ChunLiang,
Chang Tschining,
Chen ChunJung
Publication year - 2006
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309105040698
Subject(s) - oryza sativa , crystallization , lectin , crystallography , x ray , chemistry , materials science , biochemistry , organic chemistry , physics , gene , quantum mechanics
Lectins with sugar‐binding specificity are widely distributed in higher plants and various other species. The expression of rice lectin from Oryza sativa is up‐regulated in the growing coleoptile when anaerobic stress persists. A rice lectin of molecular weight 15.2 kDa has been crystallized using the hanging‐drop vapour‐diffusion method. From the diffraction of the lectin crystals at 1.93 Å resolution, the unit cell belongs to space group P 3 1 , with unit‐cell parameters a = 98.58, b = 98.58, c = 44.72 Å. Preliminary analysis indicates that there are two lectin molecules in an asymmetric unit with a large solvent content, 70.1%.