Open AccessOverexpression, purification and crystallization of an archaeal DNA ligase from Pyrococcus furiosus
Author(s) -
Nishida Hirokazu,
Tsuchiya Daisuke,
Ishino Yoshizumi,
Morikawa Kosuke
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309105038649
Subject(s) - pyrococcus furiosus , dna ligase , dna ligases , dna , dna replication , biology , chemistry , microbiology and biotechnology , crystallography , biochemistry , archaea , gene
DNA ligases seal single‐strand breaks in double‐stranded DNA and their function is essential to maintain the integrity of the genome during various aspects of DNA metabolism, such as replication, excision repair and recombination. DNA‐strand breaks are frequently generated as reaction intermediates in these events and the sealing of these breaks depends solely on the proper function of DNA ligase. Crystals of the archaeal DNA ligase from Pyrococcus furiosus were obtained using 6.6%( v / v ) ethanol as a precipitant and diffracted X‐rays to 1.7 Å resolution. They belong to the monoclinic space group P 2 1 , with unit‐cell parameters a = 61.1, b = 88.3, c = 63.4 Å, β = 108.9°. The asymmetric unit contains one ligase molecule.