Open AccessCrystallization and preliminary X‐ray crystallographic studies of fatty acid‐CoA racemase from Mycobacterium tuberculosis H37Rv
Author(s) -
Rhee KyungHee,
Lee Ki Seog,
Kim Eunice Eunkyung,
Priyadarshi Amit,
Hwang Kwang Yeon
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309105034330
Subject(s) - crystallization , mycobacterium tuberculosis , crystallography , tuberculosis , chemistry , materials science , medicine , organic chemistry , pathology
Fatty acid‐CoA racemase plays an important role in the β‐oxidation of branched‐chain fatty acids and fatty‐acid derivatives as it catalyzes the conversion of several (2 R )‐branched‐chain fatty acid‐CoAs to their (2 S )‐stereoisomers. Fatty acid‐CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging‐drop vapour‐diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P 3 1 or P 3 2 , with unit‐cell parameters a = b = 109.56, c = 147.97 Å. The asymmetric unit contains six monomers, corresponding to a V M value of 2.15 Å 3 Da −1 . A complete native data set has been collected at 2.7 Å resolution using a synchrotron‐radiation source.