Open AccessCrystallization and preliminary X‐ray crystallographic analysis of Sulfolobus solfataricus thioredoxin reductase
Author(s) -
Ruggiero Alessia,
Ruocco Maria Rosaria,
Grimaldi Pasquale,
Arcari Paolo,
Masullo Mariorosario,
Zagari Adriana,
Vitagliano Luigi
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309105027995
Subject(s) - sulfolobus solfataricus , sulfolobus , crystallography , crystallization , thioredoxin reductase , x ray , materials science , chemistry , enzyme , biochemistry , thioredoxin , physics , archaea , optics , organic chemistry , gene
A thermostable thioredoxin reductase isolated from Sulfolobus solfataricus ( Ss TrxR) has been successfully crystallized in the absence and in the presence of NADP. Two different crystal forms have been obtained. Crystals of the form that yields higher resolution data (1.8 Å) belong to space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 76.77, b = 120.68, c = 126.85 Å. The structure of the enzyme has been solved by MAD methods using the anomalous signal from the Se atoms of selenomethionine‐labelled Ss TrxR.