Open AccessCloning, purification, crystallization and preliminary X‐ray crystallographic analysis of XC847, a 3′‐5′ oligoribonuclease from Xanthomonas campestris
Author(s) -
Wu YanYou,
Chin KoHsin,
Chou ChiaCheng,
Lee ChengChung,
Shr HuiLin,
Gao Fei Philip,
Lyu PingChiang,
Wang Andrew H.J.,
Chou ShanHo
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309105027132
Subject(s) - xanthomonas campestris , cloning (programming) , biology , gene , crystallization , xanthomonas , bacteria , tetragonal crystal system , microbiology and biotechnology , genetics , chemistry , crystallography , organic chemistry , computer science , crystal structure , programming language
Oligoribonucleases are essential components of RNA and DNA metabolism and close homologues of genes encoding them are found not only in prokaryotes but also in a wide range of eukaryotes, including yeast and humans. Inactivation of the oligoribonuclease gene ( orn ) can result in cellular lethality. Despite their important biological function, they have been studied little from a structural point of view. In this report, the cloning, expression, crystallization and preliminary X‐ray analysis of XC847, a DEDDh‐type 3′‐5′ oligoribonuclease from the plant pathogen Xanthomonas campestris pv. campestris , a Gram‐negative bacterium causing major worldwide disease of cruciferous crops, is described. The XC847 crystals diffracted to a resolution of at least 2.1 Å. They are tetragonal and belong to space group P 4 3 2 1 2, with unit‐cell parameters a = b = 67.5, c = 89.8 Å. One molecule is present per asymmetric unit.