Open AccessExpression, crystallization and preliminary X‐ray analysis of the extracellular Ig modules I–IV and F3 modules I–III of the neural cell‐adhesion molecule L1
Author(s) -
Bock Elisabeth,
Kasper Christina,
Kastrup Jette Sandholm,
Kulahin Nikolaj,
Berezin Vladimir,
Kristensen Ole,
Gajhede Michael
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309105026783
Subject(s) - crystallization , extracellular , molecule , cell adhesion molecule , adhesion , crystallography , materials science , neural cell adhesion molecule , cell adhesion , chemistry , biophysics , microbiology and biotechnology , biology , biochemistry , composite material , organic chemistry
Four amino‐terminal immunoglobulin (Ig) modules and three fibronectin type III (F3) modules of the mouse neural cell‐adhesion molecule L1 have been expressed in Drosophila S2 cells. The Ig modules I–IV of L1 crystallized in a trigonal space group, with unit‐cell parameters a = b = 239.6, c = 99.3 Å, and the crystals diffracted X‐rays to a resolution of about 3.5 Å. The F3 modules I–III of L1 crystallized in a tetragonal space group, with unit‐cell parameters a = b = 80.1, c = 131 Å, and the crystals diffracted X‐rays to 2.8 Å resolution. This is a step towards the structure determination of the multimodular constructs of the neural cell‐adhesion molecule L1 in order to understand the function of L1 on a structural basis.