Open AccessCrystallization and preliminary crystallographic analysis of the (cytosine‐5)‐DNA methyltransferase NlaX from Neisseria lactamica
Author(s) -
Artyukh Rimma I.,
Lavrova Natalia V.,
Kachalova Galina S.,
Ryazanova Elena M.,
Karyagina Anna S.,
Kubareva Elena A.,
Bartunik Hans D.
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309105026709
Subject(s) - cytosine , crystallization , crystallography , resolution (logic) , methyltransferase , chemistry , dna methyltransferase , dna , molecule , materials science , biochemistry , methylation , organic chemistry , artificial intelligence , computer science
Crystals of the (cytosine‐5)‐DNA methyltransferase NlaX from Neisseria lactamica (molecular weight 36.5 kDa) have been grown at 291 K using 2.5 M NaCl as precipitant. The crystals diffract to 3.0 Å resolution at 100 K. The crystals belong to space group P 321, with unit‐cell parameters a = 121.98, b = 121.98, c = 56.71 Å. There is one molecule in the asymmetric unit and the solvent content is estimated to be 62.1% by volume.