Open AccessCrystallization and preliminary X‐ray analysis of the YjgF/YER057c/UK114‐family protein ST0811 from Sulfolobus tokodaii strain 7
Author(s) -
Hatano Kenichi,
Miyakawa Takuya,
Lee Woo Cheol,
Kato Yusuke,
Sawano Yoriko,
Yumoto Fumiaki,
Nagata Koji,
Tanokura Masaru
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309105023808
Subject(s) - crystallography , crystallization , materials science , strain (injury) , hexagonal crystal system , solvent , chemistry , biology , organic chemistry , anatomy
ST0811 from Sulfolobus tokodaii strain 7, a member of the YjgF/YER057c/UK114 protein family, was crystallized by the sitting‐drop vapour‐diffusion method using PEG 10 000 as precipitant. The crystals diffracted X‐rays to beyond 2.0 Å resolution using an in‐house rotating‐anode generator. The crystals belonged to the rhombohedral space group R 3, with hexagonal unit‐cell parameters a = b = 55.0, c = 223.2 Å. The crystals contained two molecules in the asymmetric unit ( V M = 2.3 Å 3 Da −1 ) and had a solvent content of 47%.