Open AccessPurification, crystallization and preliminary crystallographic studies of a calmodulin‐OLFp hybrid molecule
Author(s) -
Chyan ChiaLin,
Huang PoChung,
Lin TaHsien,
Huang JianWen,
Lin S. S.,
Huang Hsienbin,
Chen YiCheng
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309105023006
Subject(s) - crystallization , calmodulin , molecule , crystallography , materials science , chemistry , calcium , metallurgy , organic chemistry
A hybrid molecule consisting of calmodulin (CaM) and the CaM‐binding domain of olfactory nucleotide‐gated ion‐channel peptide (CaM‐OLFp) was purified and crystallized by the hanging‐drop vapour‐diffusion method at 298 K. The crystals diffracted to a maximum resolution of 1.85 Å at cryogenic temperature (100 K) using X‐rays from a rotating anode (Cu, wavelength 1.54 Å). The crystal belongs to the monoclinic space group C 2, with unit‐cell parameters a = 64.76, b = 36.23, c = 70.96 Å, α = γ = 90, β = 109.4°. Analysis of the packing density shows that the asymmetric unit contains one CaM‐OLFp hybrid molecule with a solvent content of 36.42%.