Open AccessOligonucleotides with cyclohexene‐nucleoside building blocks: crystallization and preliminary X‐ray studies of a left‐handed sequence GTGTACAC
Author(s) -
Robeyns Koen,
Herdewijn Piet,
Van Meervelt Luc
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309105015228
Subject(s) - cyclohexene , oligonucleotide , deoxyribose , phosphoramidite , crystallography , chemistry , sequence (biology) , nucleic acid , crystallization , stereochemistry , dna , organic chemistry , biochemistry , catalysis
Cyclohexene nucleic acids contain a cyclohexene ring instead of the normal β‐ d ‐2′‐deoxyribose. The cyclohexene oligonucleotide GTGTACAC was synthesized using phosphoramidite chemistry and standard protecting groups. Crystals of GTGTACAC were obtained at 289 K by the hanging‐drop vapour‐diffusion technique. The crystals diffract to 1.7 Å resolution and belong to the trigonal space group R 3, with unit‐cell parameters a = 41.434, c = 66.735 Å.