Open AccessCrystallization and preliminary X‐ray characterization of a ferritin from the hyperthermophilic archaeon and anaerobe Pyrococcus furiosus
Author(s) -
Matias Pedro M.,
Tatur Jana,
Carrondo Maria Arménia,
Hagen Wilfred R.
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309105011516
Subject(s) - pyrococcus furiosus , orthorhombic crystal system , crystallography , crystallization , monomer , chemistry , ferritin , thermophile , materials science , crystal structure , biochemistry , enzyme , archaea , organic chemistry , gene , polymer
Crystals of the title protein have been produced and preliminary structural analysis has been carried out. The crystals belong to the orthorhombic space group C 222 1 , with unit‐cell parameters a = 258.1, b = 340.1, c = 266.5 Å. The protein forms a 24‐mer of 20 kDa subunits, which assemble with 432 non‐crystallographic symmetry. A total of 36 monomers are found in the asymmetric unit, corresponding to one and a half 24‐mers.