Open AccessExpression, purification, crystallization and preliminary X‐ray analysis of YaeQ (XAC2396) from Xanthomonas axonopodis pv. citri
Author(s) -
Guzzo Cristiane R.,
Nagem Ronaldo A. P.,
GalvãoBotton Leonor M. P.,
Guimarães Beatriz G.,
Medrano Francisco J.,
Barbosa João A. R. G.,
Farah Chuck S.
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309105010985
Subject(s) - crystallization , recombinant dna , xanthomonas , cloning (programming) , resolution (logic) , crystallography , diffraction , biology , microbiology and biotechnology , chemistry , physics , optics , bacteria , genetics , gene , organic chemistry , artificial intelligence , computer science , programming language
Xanthomonas axonopodis pv. citri YaeQ (XAC2396) is a member of a family of bacterial proteins conserved in several Gram‐negative pathogens. Here, the cloning, expression, purification and crystallization of the 182‐residue (20.6 kDa) YaeQ protein are described. Recombinant YaeQ containing selenomethionine was crystallized in space group P 2 1 and crystals diffracted to 1.9 Å resolution at a synchrotron source. The unit‐cell parameters are a = 39.75, b = 91.88, c = 48.03 Å, β = 108.37°. The calculated Matthews coefficient suggests the presence of two YaeQ molecules in the asymmetric unit. Initial experimental phases were calculated by the multiple‐wavelength anomalous dispersion technique and an interpretable electron‐density map was obtained.