Open AccessCrystallization and preliminary X‐ray analysis of a novel thermoalkalophilic poly(3‐hydroxybutyrate) depolymerase (PhaZ7) from Paucimonas lemoignei
Author(s) -
Braaz Reinhard,
Kapetaniou Evangelia G.,
Jendrossek Dieter,
Papageorgiou Anastassios C.
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s174430910501016x
Subject(s) - polyhydroxyalkanoates , crystallization , polyester , substrate (aquarium) , materials science , amorphous solid , methacrylate , poly 3 hydroxybutyrate , monoclinic crystal system , polymer chemistry , chemistry , polymer , chemical engineering , nuclear chemistry , bacteria , organic chemistry , monomer , molecule , biochemistry , biology , ecology , genetics , engineering
Polyhydroxyalkanoates (PHA) are biodegradable polyesters that have attracted commercial and academic interest as environmentally friendly materials. A number of enzymes are able to degrade polyhydroxyalkanoates to water‐soluble products. PhaZ7 poly(3‐hydroxybutyrate) (PHB) depolymerase (EC 3.1.1.75), a 342‐amino‐acid hydrolase from the PHA‐degrading bacterium Paucimonas lemoignei , has been found to possess substrate specificity for amorphous PHA. PhaZ7 was crystallized by the microdialysis method. Thin rod‐like crystals were grown in low ionic strength solution and found to belong to the monoclinic space group C 2, with unit‐cell parameters a = 225.8, b = 46.5, c = 171.3, β = 128.9°. A complete data set was collected to 2.75 Å resolution at 100 K using synchrotron radiation.