Open AccessCrystallization and preliminary X‐ray diffraction analysis of a chitin‐binding domain of hyperthermophilic chitinase from Pyrococcus furiosus
Author(s) -
Oku Takashi,
Nakamura Tsutomu,
Inoue Tsuyoshi,
Ataka Mitsuo,
Ishikawa Kazuhiko,
Hagihara Yoshihisa,
Nakagawa Atsushi,
Uegaki Koichi
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309105010110
Subject(s) - pyrococcus furiosus , chitin , chitinase , crystallization , crystallography , resolution (logic) , x ray crystallography , chemistry , diffraction , escherichia coli , materials science , biochemistry , archaea , optics , organic chemistry , physics , enzyme , gene , chitosan , artificial intelligence , computer science
The crystallization and preliminary X‐ray diffraction analysis of the chitin‐binding domain of chitinase from a hyperthermophilic archaeon, Pyrococcus furiosus , are reported. The recombinant protein was prepared using an Escherichia coli overexpression system and was crystallized by the hanging‐drop vapour‐diffusion method. An X‐ray diffraction data set was collected to 1.70 Å resolution. The crystal belonged to space group P 4 3 2 1 2 or P 4 1 2 1 2. The unit‐cell parameters were determined to be a = b = 48.8, c = 85.0 Å.