Open AccessExpression, purification, crystallization and preliminary X‐ray analysis of Aeromonas hydrophilia metallo‐β‐lactamase
Author(s) -
Sharma Nandini,
Toney Jeffrey H.,
Fitzgerald Paula M. D.
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309104033512
Subject(s) - crystallization , orthorhombic crystal system , monomer , solvent , ammonium sulfate , aeromonas , chemistry , crystallography , nuclear chemistry , chromatography , bacteria , crystal structure , polymer , biochemistry , biology , organic chemistry , genetics
The CphA metallo‐β‐lactamase from Aeromonas hydrophilia has been expressed, purified and crystallized by the hanging‐drop vapor‐diffusion method using ammonium sulfate as the precipitant. The crystals exhibit orthorhombic symmetry ( P 2 1 2 1 2), with unit‐cell parameters a = 40.75, b = 42.05, c = 128.88 Å. There is one monomer in the asymmetric unit and the solvent content is estimated to be 44% by volume. A data set extending to 1.8 Å has been measured.