Expression, purification, crystallization and preliminary X‐ray analysis of Aeromonas hydrophilia metallo‐β‐lactamase

The CphA metallo‐β‐lactamase from Aeromonas hydrophilia has been expressed, purified and crystallized by the hanging‐drop vapor‐diffusion method using ammonium sulfate as the precipitant. The crystals exhibit orthorhombic symmetry ( P 2 1 2 1 2), with unit‐cell parameters a = 40.75, b = 42.05, c = 128.88 Å. There is one monomer in the asymmetric unit and the solvent content is estimated to be 44% by volume. A data set extending to 1.8 Å has been measured.