Crystallization and preliminary X‐ray diffraction analysis of human growth and differentiation factor 5 (GDF‐5) | Zendy

Mueller Thomas D. | Zendy; Gottermeier Maike | Zendy; Sebald Walter | Zendy; Nickel Joachim | Zendy

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Crystallization and preliminary X‐ray diffraction analysis of human growth and differentiation factor 5 (GDF‐5)

Author(s) -

Mueller Thomas D.,

Gottermeier Maike,

Sebald Walter,

Nickel Joachim

Publication year - 2005

Publication title -

acta crystallographica section f

Language(s) - English

Resource type - Journals

ISSN - 1744-3091

DOI - 10.1107/s1744309104031963

Subject(s) - crystallization , materials science , x ray crystallography , growth differentiation factor , crystallography , diffraction , chemistry , optics , physics , biochemistry , organic chemistry , gene , bone morphogenetic protein

Growth and differentiation factor 5 (GDF‐5) belongs to the large TGF‐β superfamily of secreted signalling proteins and plays a pivotal role in skeletal development during embryogenesis. The gene for human GDF‐5 was cloned, expressed in Escherichia coli and purified to homogeneity. Crystals were obtained that diffracted to 2.2 Å resolution. A native data set was acquired, showing that the crystals belong to a trigonal space group, i.e. P 3 1 21 or P 3 2 21, with unit‐cell parameters a = b = 97.1, c = 48.3 Å. Initial analysis suggest the presence of only one monomer in the asymmetric unit, resulting in a high solvent content of 72% in the crystal.

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