Open AccessCrystallization and preliminary X‐ray crystallographic analysis of osteoclast‐stimulating factor
Author(s) -
Li Ming,
Meng Zhaohui,
Xu Yanhui,
Lou Zhiyong,
Rao Zihe
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309104031653
Subject(s) - osteoclast , orthorhombic crystal system , crystallization , ammonium sulfate , crystallography , bone resorption , chemistry , materials science , crystal structure , biochemistry , biology , endocrinology , in vitro , chromatography , organic chemistry
Osteoclast‐stimulating factor increases osteoclast formation and bone resorption through a cellular signal transduction cascade, possibly by its interaction with c‐Src or related family members. Crystals of human osteoclast‐stimulating factor were obtained by the hanging‐drop vapour‐diffusion method using ammonium sulfate as a precipitant. The crystals are primitive orthorhombic and belong to P 222 or a related space group, with unit‐cell parameters a = 38.1, b = 54.9, c = 64.7 Å.