Open AccessCrystallization and preliminary X‐ray diffraction studies of a catechol‐ O ‐methyltransferase/inhibitor complex
Author(s) -
Rodrigues M. L.,
Bonifácio M. J.,
SoaresdaSilva P.,
Carrondo M. A.,
Archer M.
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309104031197
Subject(s) - crystallization , catechol , catechol o methyl transferase , crystallography , x ray crystallography , methyltransferase , materials science , chemistry , diffraction , physics , biochemistry , optics , organic chemistry , dna , gene , allele , methylation
Inhibitors of the enzyme catechol‐ O ‐methyltransferase (COMT) are used as co‐adjuvants in the therapy of Parkinson's disease. A recombinant form of the soluble cytosolic COMT from rat has been co‐crystallized with a new potent inhibitor, BIA 8‐176 [(3,4‐dihydroxy‐2‐nitrophenyl)phenylmethanone], by the vapour‐diffusion method using PEG 6K as precipitant. Crystals diffract to 1.6 Å resolution on a synchrotron‐radiation source and belong to the monoclinic space group P 2 1 , with unit‐cell parameters a = 52.77, b = 79.63, c = 61.54 Å, β = 91.14°.