Open AccessCrystallization and preliminary X‐ray crystallographic study of disproportionating enzyme from potato
Author(s) -
Imamura Kayo,
Matsuura Takanori,
Ye Zhengmao,
Takaha Takeshi,
Fujii Kazutoshi,
Kusunoki Masami,
Nitta Yasunori
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309104030829
Subject(s) - crystallization , crystallography , intramolecular force , intermolecular force , enzyme , crystal (programming language) , chemistry , x ray , crystal structure , resolution (logic) , materials science , stereochemistry , molecule , biochemistry , physics , organic chemistry , optics , artificial intelligence , computer science , programming language
Disproportionating enzyme (D‐enzyme; EC 2.4.1.25) is a 59 kDa protein that belongs to the α‐amylase family. D‐enzyme catalyses intramolecular and intermolecular transglycosylation reactions of α‐1,4 glucan. A crystal of the D‐enzyme from potato was obtained by the hanging‐drop vapour‐diffusion method. Preliminary X‐ray data showed that the crystal diffracts to 2.0 Å resolution and belongs to space group C 222 1 , with unit‐cell parameters a = 69.7, b = 120.3, c = 174.2 Å.
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