Open AccessCrystallization and preliminary X‐ray analysis of gene product 44 from bacteriophage Mu
Author(s) -
Kondou Youhei,
Kitazawa Daisuke,
Takeda Shigeki,
Yamashita Eiki,
Mizuguchi Mineyuki,
Kawano Keiichi,
Tsukihara Tomitake
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309104029574
Subject(s) - beamline , resolution (logic) , crystallization , crystallography , x ray , bacteriophage , diffraction , gene product , gene , x ray crystallography , product (mathematics) , materials science , optics , chemistry , biology , physics , beam (structure) , escherichia coli , genetics , gene expression , geometry , organic chemistry , artificial intelligence , computer science , mathematics
Bacteriophage Mu baseplate protein gene product 44 (gp44) is an essential protein required for the assembly of viable phages. To investigate the roles of gp44 in baseplate assembly and infection, gp44 was crystallized at pH 6.0 in the presence of 20% 2‐methyl‐2,4‐pentanediol. The crystals belong to space group R 3, with unit‐cell parameters a = b = 127.47, c = 63.97 Å. The crystals diffract X‐rays to at least 2.1 Å resolution and are stable in the X‐ray beam and are therefore appropriate for structure determination. Native data have been collected to 2.1 Å resolution using a DIP6040 image‐plate system at beamline BL44XU at the SPring‐8 facility in Japan.