Open AccessFixed‐target serial crystallography at the Structural Biology Center
Author(s) -
Sherrell Darren A.,
Lavens Alex,
Wilamowski Mateusz,
Kim Youngchang,
Chard Ryan,
Lazarski Krzysztof,
Rosenbaum Gerold,
Vescovi Rafael,
Johnson Jessica L.,
Akins Chase,
Chang Changsoo,
Michalska Karolina,
Babnigg Gyorgy,
Foster Ian,
Joachimiak Andrzej
Publication year - 2022
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s1600577522007895
Subject(s) - synchrotron radiation , synchrotron radiation source , materials science , stack (abstract data type) , diffraction , crystallography , optics , computer science , physics , chemistry , programming language
Serial synchrotron crystallography enables the study of protein structures under physiological temperature and reduced radiation damage by collection of data from thousands of crystals. The Structural Biology Center at Sector 19 of the Advanced Photon Source has implemented a fixed‐target approach with a new 3D‐printed mesh‐holder optimized for sample handling. The holder immobilizes a crystal suspension or droplet emulsion on a nylon mesh, trapping and sealing a near‐monolayer of crystals in its mother liquor between two thin Mylar films. Data can be rapidly collected in scan mode and analyzed in near real‐time using piezoelectric linear stages assembled in an XYZ arrangement, controlled with a graphical user interface and analyzed using a high‐performance computing pipeline. Here, the system was applied to two β‐lactamases: a class D serine β‐lactamase from Chitinophaga pinensis DSM 2588 and L1 metallo‐β‐lactamase from Stenotrophomonas maltophilia K279a.