Open AccessID23‐2: an automated and high‐performance microfocus beamline for macromolecular crystallography at the ESRF
Author(s) -
Nanao Max,
Basu Shibom,
Zander Ulrich,
Giraud Thierry,
Surr John,
Guijarro Matias,
Lentini Mario,
Felisaz Franck,
Sinoir Jeremy,
Morawe Christian,
Vivo Amparo,
Beteva Antonia,
Oscarsson Marcus,
Caserotto Hugo,
Dobias Fabien,
Flot David,
Nurizzo Didier,
Gigmes Jonathan,
Foos Nicolas,
Siebrecht Ralf,
Roth Thomas,
Theveneau Pascal,
Svensson Olof,
Papp Gergely,
Lavault Bernard,
Cipriani Florent,
Barrett Ray,
Clavel Carole,
Leonard Gordon
Publication year - 2022
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s1600577522000984
Subject(s) - beamline , microbeam , synchrotron radiation , synchrotron , optics , diffractometer , upgrade , sample (material) , high energy x rays , materials science , data reduction , physics , computer science , scanning electron microscope , beam (structure) , data mining , thermodynamics , operating system
ID23‐2 is a fixed‐energy (14.2 keV) microfocus beamline at the European Synchrotron Radiation Facility (ESRF) dedicated to macromolecular crystallography. The optics and sample environment have recently been redesigned and rebuilt to take full advantage of the upgrade of the ESRF to the fourth generation Extremely Brilliant Source (ESRF‐EBS). The upgraded beamline now makes use of two sets of compound refractive lenses and multilayer mirrors to obtain a highly intense (>10 13 photons s −1 ) focused microbeam (minimum size 1.5 µm × 3 µm full width at half‐maximum). The sample environment now includes a FLEX‐HCD sample changer/storage system, as well as a state‐of‐the‐art MD3Up high‐precision multi‐axis diffractometer. Automatic data reduction and analysis are also provided for more advanced protocols such as synchrotron serial crystallographic experiments.