Open AccessDetection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopy
Author(s) -
Estève Emmanuel,
Buob David,
Jamme Frédéric,
Jouanneau Chantal,
Kascakova Slavka,
Haymann Jean-Philippe,
Letavernier Emmanuel,
Galmiche Louise,
Ronco Pierre,
Daudon Michel,
Bazin Dominique,
Réfrégiers Matthieu
Publication year - 2022
Publication title -
journal of synchrotron radiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 99
ISSN - 1600-5775
DOI - 10.1107/s1600577521011371
Subject(s) - calcium oxalate , synchrotron , calcium oxalate crystals , oxalate , fluorescence , chemistry , beamline , kidney , renal function , creatinine , kidney stones , pathology , medicine , urology , biochemistry , optics , inorganic chemistry , beam (structure) , physics
Renal oxalosis is a rare cause of renal failure whose diagnosis can be challenging. Synchrotron deep ultraviolet (UV) fluorescence was assayed to improve oxalosis detection on kidney biopsies spatial resolution and sensitivity compared with the Fourier transform infrared microspectroscopy gold standard. The fluorescence spectrum of synthetic mono‐, di‐ and tri‐hydrated calcium oxalate was investigated using a microspectrometer coupled to the synchrotron UV beamline DISCO, Synchrotron SOLEIL, France. The obtained spectra were used to detect oxalocalcic crystals in a case control study of 42 human kidney biopsies including 19 renal oxalosis due to primary (PHO, n = 11) and secondary hyperoxaluria (SHO, n = 8), seven samples from PHO patients who received combined kidney and liver transplants, and 16 controls. For all oxalocalcic hydrates samples, a fluorescence signal is detected at 420 nm. These spectra were used to identify standard oxalocalcic crystals in patients with PHO or SHO. They also revealed micrometric crystallites as well as non‐aggregated oxalate accumulation in tubular cells. A nine‐points histological score was established for the diagnosis of renal oxalosis with 100% specificity (76–100) and a 73% sensitivity (43–90). Oxalate tubular accumulation and higher histological score were correlated to lower estimated glomerular filtration rate and higher urinary oxalate over creatinine ratio.