Open AccessInsights into the binding of PARP inhibitors to the catalytic domain of human tankyrase‐2
Author(s) -
Qiu Wei,
Lam Robert,
Voytyuk Oleksandr,
Romanov Vladimir,
Gordon Roni,
Gebremeskel Simon,
Vodsedalek Jakub,
Thompson Christine,
Beletskaya Irina,
Battaile Kevin P.,
Pai Emil F.,
Rottapel Robert,
Chirgadze Nickolay Y.
Publication year - 2014
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
ISSN - 1399-0047
DOI - 10.1107/s1399004714017660
Subject(s) - poly adp ribose polymerase , parp1 , polymerase , cancer research , parp inhibitor , chemistry , computational biology , rational design , biology , microbiology and biotechnology , dna , biochemistry , genetics
The poly(ADP‐ribose) polymerase (PARP) family represents a new class of therapeutic targets with diverse potential disease indications. PARP1 and PARP2 inhibitors have been developed for breast and ovarian tumors manifesting double‐stranded DNA‐repair defects, whereas tankyrase 1 and 2 (TNKS1 and TNKS2, also known as PARP5a and PARP5b, respectively) inhibitors have been developed for tumors with elevated β‐catenin activity. As the clinical relevance of PARP inhibitors continues to be actively explored, there is heightened interest in the design of selective inhibitors based on the detailed structural features of how small‐molecule inhibitors bind to each of the PARP family members. Here, the high‐resolution crystal structures of the human TNKS2 PARP domain in complex with 16 various PARP inhibitors are reported, including the compounds BSI‐201, AZD‐2281 and ABT‐888, which are currently in Phase 2 or 3 clinical trials. These structures provide insight into the inhibitor‐binding modes for the tankyrase PARP domain and valuable information to guide the rational design of future tankyrase‐specific inhibitors.