Open AccessCrystallization and preliminary crystallographic data for the augmenter of liver regeneration
Author(s) -
Rose J. P.,
Wu C.K.,
Francavilla A.,
Prelich J. G.,
Iacobellis A.,
Hagiya M.,
Rao A. S.,
Starzl T. E.,
Wang B.C.
Publication year - 1997
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
ISSN - 1399-0047
DOI - 10.1107/s0907444996014084
Subject(s) - crystallization , regeneration (biology) , liver regeneration , chemistry , gene , molecule , peripheral blood mononuclear cell , microbiology and biotechnology , crystallography , biology , biochemistry , in vitro , organic chemistry
A new cellular growth factor termed augmenter of liver regeneration (ALR) has been crystallized. ALR has been shown to have a proliferative effect on liver cells while at the same time producing an immunosuppressive effect on liver‐resident natural killer cells and liver‐resident mononuclear leukocytes. In addition, ALR appears to play an important role in the synthesis and stabilization of mitochondrial gene transcripts in actively regenerating cells. ALR crystals diffract to beyond 2 Å resolution and belong to space group P 2 1 2 1 2, with a = 125.1, b = 108.1 and c = 38.5 Å. Based on four molecules per asymmetric unit, the Matthews coefficient is calculated to be 2.16 Å 3 Da −1 which corresponds to a solvent content of 43%.