Open AccessUsing high‐throughput in situ plate screening to evaluate the effect of dehydration on protein crystals
Author(s) -
Douangamath Alice,
Aller Pierre,
Lukacik Petra,
SanchezWeatherby Juan,
Moraes Isabel,
BrandaoNeto Jose
Publication year - 2013
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
ISSN - 1399-0047
DOI - 10.1107/s0907444913002412
Subject(s) - dehydration , crystallization , throughput , in situ , diffraction , protein crystallization , crystal (programming language) , materials science , chemical engineering , computer science , chemistry , physics , optics , engineering , organic chemistry , biochemistry , telecommunications , wireless , programming language
Crystal dehydration is a post‐crystallization technique that can potentially improve the diffraction of macromolecular crystals. There are currently several ways of undertaking this process; however, dehydration experiments are often limited in their throughput and require prior manipulation of the samples. In the present study, a novel method is proposed that uses in situ plate screening to assess the effect of dehydration by combining the throughput of 96‐well crystallization plates with direct X‐ray feedback on crystal diffraction quality.