Open AccessNanoflow electrospinning serial femtosecond crystallography
Author(s) -
Sierra Raymond G.,
Laksmono Hartawan,
Kern Jan,
Tran Rosalie,
Hattne Johan,
AlonsoMori Roberto,
LassalleKaiser Benedikt,
Glöckner Carina,
Hellmich Julia,
Schafer Donald W.,
Echols Nathaniel,
Gildea Richard J.,
GrosseKunstleve Ralf W.,
Sellberg Jonas,
McQueen Trevor A.,
Fry Alan R.,
Messerschmidt Marc M.,
Miahnahri Alan,
Seibert M. Marvin,
Hampton Christina Y.,
Starodub Dmitri,
Loh N. Duane,
Sokaras Dimosthenis,
Weng TsuChien,
Zwart Petrus H.,
Glatzel Pieter,
Milathianaki Despina,
White William E.,
Adams Paul D.,
Williams Garth J.,
Boutet Sébastien,
Zouni Athina,
Messinger Johannes,
Sauter Nicholas K.,
Bergmann Uwe,
Yano Junko,
Yachandra Vittal K.,
Bogan Michael J.
Publication year - 2012
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
ISSN - 1399-0047
DOI - 10.1107/s0907444912038152
Subject(s) - femtosecond , electrospinning , diffraction , thermolysin , resolution (logic) , materials science , laser , x ray crystallography , crystallography , chemistry , optics , composite material , physics , trypsin , polymer , computer science , organic chemistry , artificial intelligence , enzyme
An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14–3.1 µl min −1 to perform serial femtosecond crystallography (SFX) studies with X‐ray lasers. Thermolysin microcrystals flowed at 0.17 µl min −1 and diffracted to beyond 4 Å resolution, producing 14 000 indexable diffraction patterns, or four per second, from 140 µg of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.