Open AccessPseudo‐merohedral twinning and noncrystallographic symmetry in orthorhombic crystals of SIVmac239 Nef core domain bound to different‐length TCRζ fragments
Author(s) -
Kim Walter M.,
Sigalov Alexander B.,
Stern Lawrence J.
Publication year - 2010
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
ISSN - 1399-0047
DOI - 10.1107/s090744490904880x
Subject(s) - crystal twinning , crystallography , orthorhombic crystal system , crystallization , tetragonal crystal system , crystal structure , crystal (programming language) , chemistry , topology (electrical circuits) , materials science , mathematics , combinatorics , computer science , microstructure , organic chemistry , programming language
HIV/SIV Nef mediates many cellular processes through interactions with various cytoplasmic and membrane‐associated host proteins, including the signalling ζ subunit of the T‐cell receptor (TCRζ). Here, the crystallization strategy, methods and refinement procedures used to solve the structures of the core domain of the SIVmac239 isolate of Nef (Nef core ) in complex with two different TCRζ fragments are described. The structure of SIVmac239 Nef core bound to the longer TCRζ polypeptide (Leu51–Asp93) was determined to 3.7 Å resolution ( R work = 28.7%) in the tetragonal space group P 4 3 2 1 2. The structure of SIVmac239 Nef core in complex with the shorter TCRζ polypeptide (Ala63–Arg80) was determined to 2.05 Å resolution ( R work = 17.0%), but only after the detection of nearly perfect pseudo‐merohedral crystal twinning and proper assignment of the orthorhombic space group P 2 1 2 1 2 1 . The reduction in crystal space‐group symmetry induced by the truncated TCRζ polypeptide appears to be caused by the rearrangement of crystal‐contact hydrogen‐bonding networks and the substitution of crystallographic symmetry operations by similar noncrystallographic symmetry (NCS) operations. The combination of NCS rotations that were nearly parallel to the twin operation ( k , h , − l ) and a and b unit‐cell parameters that were nearly identical predisposed the P 2 1 2 1 2 1 crystal form to pseudo‐merohedral twinning.