Open AccessThe effect of protein–precipitant interfaces and applied shear on the nucleation and growth of lysozyme crystals
Author(s) -
Reis Nuno M.,
Chirgadze Dimitri Y.,
Blundell Tom L.,
Mackley Malcolm R.
Publication year - 2009
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
ISSN - 1399-0047
DOI - 10.1107/s0907444909031527
Subject(s) - nucleation , lysozyme , crystallization , protein crystallization , optical microscope , shearing (physics) , crystallography , chemical engineering , materials science , kinetics , confocal microscopy , drop (telecommunication) , ethylene glycol , crystal growth , chemistry , scanning electron microscope , optics , composite material , biochemistry , organic chemistry , engineering , telecommunications , physics , quantum mechanics , computer science
This paper is concerned with the effect of protein–precipitant interfaces and externally applied shear on the nucleation and growth kinetics of hen egg‐white lysozyme crystals. The early stages of microbatch crystallization of lysozyme were explored using both optical and confocal fluorescence microscopy imaging. Initially, an antisolvent (precipitant) was added to a protein drop and the optical development of the protein–precipitant interface was followed with time. In the presence of the water‐soluble polymer poly(ethylene glycol) (PEG) a sharp interface was observed to form immediately within the drop, giving an initial clear separation between the lighter protein solution and the heavier precipitant. This interface subsequently became unstable and quickly developed within a few seconds into several unstable `fingers' that represented regions of high concentration‐gradient interfaces. Confocal microscopy demonstrated that the subsequent nucleation of protein crystals occurred preferentially in the region of these interfaces. Additional experiments using an optical shearing system demonstrated that oscillatory shear significantly decreased nucleation rates whilst extending the growth period of the lysozyme crystals. The experimental observations relating to both nucleation and growth have relevance in developing efficient and reliable protocols for general crystallization procedures and the controlled crystallization of single large high‐quality protein crystals for use in X‐ray crystallography.