Open AccessRotational order–disorder structure of fluorescent protein FP480
Author(s) -
Pletnev Sergei,
Morozova Kateryna S.,
Verkhusha Vladislav V.,
Dauter Zbigniew
Publication year - 2009
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
ISSN - 1399-0047
DOI - 10.1107/s0907444909020927
Subject(s) - randomness , tetramer , crystal structure , crystallography , lattice (music) , fluorescence , symmetry (geometry) , structural biology , protein structure , rotational symmetry , chemistry , physics , chemical physics , materials science , mathematics , optics , geometry , nuclear magnetic resonance , biochemistry , statistics , acoustics , enzyme
In the last decade, advances in instrumentation and software development have made crystallography a powerful tool in structural biology. Using this method, structural information can now be acquired from pathological crystals that would have been abandoned in earlier times. In this paper, the order–disorder (OD) structure of fluorescent protein FP480 is discussed. The structure is composed of tetramers with 222 symmetry incorporated into the lattice in two different ways, namely rotated 90° with respect to each other around the crystal c axis, with tetramer axes coincident with crystallographic twofold axes. The random distribution of alternatively oriented tetramers in the crystal creates a rotational OD structure with statistically averaged I 422 symmetry, although the presence of very weak and diffuse additional reflections suggests that the randomness is only approximate.