Open AccessThe befores and afters of molecular replacement
Author(s) -
Dodson Eleanor
Publication year - 2008
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
ISSN - 1399-0047
DOI - 10.1107/s0907444907049736
Subject(s) - molecular replacement , computer science , computational biology , homology (biology) , domain (mathematical analysis) , sequence (biology) , matching (statistics) , biology , protein structure , mathematics , genetics , gene , biochemistry , mathematical analysis , statistics
This review addresses the essential questions to consider when attempting to phase a new crystal structure using molecular replacement. Sequence matching can suggest whether there is a suitable three‐dimensional model available, but it is also important to analyse the model in order to find its likely oligomeric state and to establish whether there are likely to be domain movements. Once a solution has been found it must be refined, which can be challenging for low‐homology models. There is a detailed discussion of structures used as examples for CCP4 tutorials.