Open AccessApplication of the use of high‐throughput technologies to the determination of protein structures of bacterial and viral pathogens
Author(s) -
Fogg M. J.,
Bahar M.,
Alzari P.,
Bertini I.,
Betton J.M.,
Burmeister W. P.,
Cambillau C.,
Coll M.,
Canard B.,
Carrondo M.,
Daenke S.,
Dym O.,
Geerlof A.,
Egloff M.P.,
Haouz A.,
Enguita F. J.,
Jones T. A.,
Ma Qingjun,
Manicka S. N.,
Owens R.J.,
Migliardi M.,
Nordlund P.,
Peleg Y.,
Schneider G.,
Schnell R.,
Stuart D. I.,
Unge T.,
Tarbouriech N.,
Wilkinson A. J.,
Wilmanns M.,
Wilson K. S.,
Zimhony O.,
Grimes J. M.
Publication year - 2006
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
ISSN - 1399-0047
DOI - 10.1107/s0907444906030915
Subject(s) - proteomics , throughput , computational biology , human proteins , human pathogen , bacterial protein , human health , biology , nanotechnology , computer science , bacteria , biochemistry , materials science , medicine , gene , genetics , telecommunications , environmental health , wireless
The Structural Proteomics In Europe (SPINE) programme is aimed at the development and implementation of high‐throughput technologies for the efficient structure determination of proteins of biomedical importance, such as those of bacterial and viral pathogens linked to human health. Despite the challenging nature of some of these targets, 175 novel pathogen protein structures (∼220 including complexes) have been determined to date. Here the impact of several technologies on the structural determination of proteins from human pathogens is illustrated with selected examples, including the parallel expression of multiple constructs, the use of standardized refolding protocols and optimized crystallization screens.