Open AccessFirst steps towards effective methods in exploiting high‐throughput technologies for the determination of human protein structures of high biomedical value
Author(s) -
Banci L.,
Bertini I.,
Cusack S.,
De Jong R. N.,
Heinemann U.,
Jones E. Y.,
Maskos K.,
Kozielski F.,
Owens R.,
Perrakis A.,
Siebold C.,
Silman I.,
Messerschmidt A.,
Poterszman A.,
Schneider G.,
Sixma T.,
Sussman J. L.,
StewartJones G.,
Thierry J.C.,
Moras Dino
Publication year - 2006
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
ISSN - 1399-0047
DOI - 10.1107/s0907444906029350
Subject(s) - computational biology , high resolution , kinase , throughput , proteomics , nanotechnology , biology , computer science , microbiology and biotechnology , biochemistry , materials science , gene , remote sensing , telecommunications , geology , wireless
The EC `Structural Proteomics In Europe' contract is aimed specifically at the atomic resolution structure determination of human protein targets closely linked to health, with a focus on cancer (kinesins, kinases, proteins from the ubiquitin pathway), neurological development and neurodegenerative diseases and immune recognition. Despite the challenging nature of the analysis of such targets, ∼170 structures have been determined to date. Here, the impact of high‐throughput technologies, such as parallel expression of multiple constructs, the use of standardized refolding protocols and optimized crystallization screens or the use of mass spectrometry to assist sample preparation, on the structural biology of mammalian protein targets is illustrated through selected examples.