Open AccessReproducible improvements in order and diffraction limit of crystals of bovine mitochondrial F 1 ‐ATPase by controlled dehydration
Author(s) -
Bowler Matthew W.,
Montgomery Martin G.,
Leslie Andrew G. W.,
Walker John E.
Publication year - 2006
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
ISSN - 1399-0047
DOI - 10.1107/s0907444906020877
Subject(s) - orthorhombic crystal system , diffraction , dehydration , angstrom , x ray crystallography , crystallography , synchrotron radiation , resolution (logic) , materials science , optics , chemistry , physics , biochemistry , artificial intelligence , computer science
Orthorhombic crystals of bovine F 1 ‐ATPase have been subjected to controlled dehydration. A decrease in the relative humidity surrounding the crystals to 90% reproducibly reduced their unit‐cell volume by 22% (950 000 Å 3 ) and improved the diffraction limit and mosaic spread of the crystals significantly. These dehydrated crystals diffracted X‐rays to 1.8 Å resolution at a synchrotron source, the best diffraction limit yet attained with these crystals, although radiation damage limited the resolution of a complete data set to 1.95 Å.