Open AccessSample preparation and mass‐spectrometric characterization of crystal‐derived protein samples
Author(s) -
Walter Thomas S.,
Aplin Robin,
Nettleship Joanne E.,
Stammers David K.,
Owens Ray J.
Publication year - 2005
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
ISSN - 1399-0047
DOI - 10.1107/s0907444905007262
Subject(s) - mass spectrometry , crystallization , protein crystallization , chromatography , chemistry , crystal (programming language) , characterization (materials science) , sample preparation , analytical chemistry (journal) , materials science , nanotechnology , computer science , organic chemistry , programming language
Mass spectrometry is often used to ascertain the accurate mass of purified protein samples prior to crystallization screening. However, in many cases data regarding the form of the protein crystallizing can also be useful, as this may differ from the original sample. Development of a simple method for the preparation and mass spectrometry of crystal‐derived protein samples is described. The method is exemplified by the determination of the phosphorylation state of protein in a crystal derived from a mixture of phosphorylated and unphosphorylated protein.