Crystallization and preliminary X‐ray diffraction analysis of levansucrase (LsdA) from  Gluconacetobacter diazotrophicus  SRT4 | Zendy

MartínezFleites Carlos | Zendy; Tarbouriech Nicolas | Zendy; OrtizLombardia Miguel | Zendy; Taylor Edward | Zendy; Rodríguez Armando | Zendy; Ramírez Ricardo | Zendy; Hernández Lázaro | Zendy; Davies Gideon J. | Zendy

Open Access

Crystallization and preliminary X‐ray diffraction analysis of levansucrase (LsdA) from Gluconacetobacter diazotrophicus SRT4

Author(s) -

MartínezFleites Carlos,

Tarbouriech Nicolas,

OrtizLombardia Miguel,

Taylor Edward,

Rodríguez Armando,

Ramírez Ricardo,

Hernández Lázaro,

Davies Gideon J.

Publication year - 2004

Publication title -

acta crystallographica section d

Language(s) - English

Resource type - Journals

ISSN - 1399-0047

DOI - 10.1107/s0907444903025514

Subject(s) - levansucrase , orthorhombic crystal system , chemistry , ammonium sulfate , crystallization , crystallography , chromatography , bacteria , crystal structure , organic chemistry , bacillus subtilis , biology , genetics

The endophytic bacterium Gluconacetobacter diazotrophicus SRT4 secretes a constitutively expressed levansucrase (LsdA; EC 2.4.1.10), which converts sucrose to fructo‐oligosaccharides and levan. Fully active LsdA was purified to high homogeneity by non‐denaturing reversed‐phase HPLC and was crystallized at room temperature by the hanging‐drop vapour‐diffusion method using ammonium sulfate and ethanol as precipitants. The crystals are extremely sensitive, but native data have been collected to 2.5 Å under cryogenic conditions using synchrotron radiation. LsdA crystals belong to the orthorhombic space group P 22 1 2 1 or P 2 1 2 1 2, with unit‐cell parameters a = 53.80, b = 119.39, c = 215.10 Å.

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