Open AccessMeasurement errors and their consequences in protein crystallography
Author(s) -
Borek Dominika,
Minor Wladek,
Otwinowski Zbyszek
Publication year - 2003
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
ISSN - 1399-0047
DOI - 10.1107/s0907444903020924
Subject(s) - sigma , process (computing) , computer science , quality (philosophy) , observational error , algorithm , econometrics , mathematics , physics , quantum mechanics , operating system
This article analyzes the relative impact of various types of measurement uncertainties on different stages of structure determination. The treatment of errors is an important part of the experimental process and becomes critical when data quality is barely sufficient to solve and/or answer detailed questions about the structure. The sources and types of experimental errors are described and methods of minimizing their impact are discussed. Practical calculations of sigma estimates in DENZO and SCALEPACK are presented.