Open AccessMacromolecular structure determination by cryo‐electron microscopy
Author(s) -
Saibil Helen R.
Publication year - 2000
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
ISSN - 1399-0047
DOI - 10.1107/s0907444900010787
Subject(s) - cryo electron microscopy , macromolecule , transmission electron microscopy , electron microscope , microscopy , complement (music) , electron tomography , crystallography , materials science , nanotechnology , chemistry , scanning transmission electron microscopy , physics , optics , biochemistry , complementation , gene , phenotype
Recent advances in transmission electron microscopy (EM) hardware, low‐temperature methods and image‐processing software have made cryo‐EM an important complement to X‐ray crystallography and NMR for macromolecular structure determination, particularly of large assemblies. This review provides a summary of the main advances and a survey of the capabilities of this approach.