Open AccessDirect methods and protein crystallography at low resolution
Author(s) -
Gilmore Christopher J.
Publication year - 2000
Publication title -
acta crystallographica section d
Language(s) - English
Resource type - Journals
ISSN - 1399-0047
DOI - 10.1107/s0907444900009355
Subject(s) - resolution (logic) , crystallography , chemistry , computer science , artificial intelligence
The tools of modern direct methods are examined and their limitations for solving protein structures discussed. Direct methods need atomic resolution data (1.1–1.2 Å) for structures of around 1000 atoms if no heavy atom is present. For low‐resolution data, alternative approaches are necessary and these include maximum entropy, symbolic addition, Sayre's equation, group scattering factors and electron microscopy.