Crystallization and preliminary X‐ray diffraction analysis of β‐cinnamomin, an elicitin secreted by the phytopathogenic fungus  Phytophthora cinnamomin | Zendy

Archer M. | Zendy; Rodrigues M. L. | Zendy; Aurélio M. | Zendy; Biemans R. | Zendy; Cravador A. | Zendy; Carrondo M. A. | Zendy

Open Access

Crystallization and preliminary X‐ray diffraction analysis of β‐cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomin

Author(s) -

Archer M.,

Rodrigues M. L.,

Aurélio M.,

Biemans R.,

Cravador A.,

Carrondo M. A.

Publication year - 2000

Publication title -

acta crystallographica section d

Language(s) - English

Resource type - Journals

ISSN - 1399-0047

DOI - 10.1107/s0907444900000366

Subject(s) - ammonium sulfate , crystallization , triclinic crystal system , phytophthora , crystallography , chemistry , x ray , x ray crystallography , nuclear chemistry , diffraction , crystal structure , botany , biology , chromatography , organic chemistry , physics , optics

Cinnamomin (CIN) belongs to a family of 10 kDa proteins designated as elicitins. Some of these proteins induce a hypersensitive response in diverse plant species, leading to resistance against fungal and bacterial plant pathogens. CIN was crystallized by the vapour‐diffusion method using either ammonium sulfate or polyethyleneglycol (PEG) as precipitants in solutions buffered at around pH 7. These crystals are isomorphous and belong to the triclinic space group, with unit‐cell parameters a = 31.69, b = 36.99, c = 44.09 Å, α = 76.86, β = 84.41, γ = 80.26°. A frozen crystal diffracted X‐rays beyond 1.45 Å resolution on a synchrotron‐radiation source.

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