Open AccessBayesian algorithms for recovering structure from single‐particle diffraction snapshots of unknown orientation: a comparison
Author(s) -
Moths Brian,
Ourmazd Abbas
Publication year - 2011
Publication title -
acta crystallographica section a
Language(s) - English
Resource type - Journals
eISSN - 1600-5724
pISSN - 0108-7673
DOI - 10.1107/s0108767311019611
Subject(s) - diffraction , femtosecond , algorithm , orientation (vector space) , limiting , optics , snapshot (computer storage) , photon , laser , particle (ecology) , physics , computer science , mathematics , engineering , geometry , geology , operating system , mechanical engineering , oceanography
The advent of X‐ray free‐electron lasers promises the possibility to determine the structure of individual particles such as microcrystallites, viruses and biomolecules from single‐shot diffraction snapshots obtained before the particle is destroyed by the intense femtosecond pulse. This program requires the ability to determine the orientation of the particle giving rise to each snapshot at signal levels as low as ~10 −2 photons per pixel. Two apparently different approaches have recently demonstrated this capability. Here we show they represent different implementations of the same fundamental approach, and identify the primary factors limiting their performance.