Open AccessRegulatory sequences of Arabidopsis drive reporter gene expression in nematode feeding structures.
Author(s) -
Nathalie Barthels,
F M van der Lee,
Joke C. Klap,
Oscar J. M. Goddijn,
Mansour Karimi,
Piotr S. Puzio,
Florian M. W. Grundler,
Stephan A. Ohl,
Keith Lindsey,
Lee Robertson,
W. M. Robertson,
Marc Van Montagu,
Godelieve Gheysen,
Peter C. Sijmons
Publication year - 1997
Publication title -
the plant cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.324
H-Index - 341
eISSN - 1532-298X
pISSN - 1040-4651
DOI - 10.1105/tpc.9.12.2119
Subject(s) - biology , arabidopsis , nematode , reporter gene , gene , effector , gus reporter system , transgene , gene expression , genetics , root knot nematode , regulation of gene expression , promoter , nematode infection , microbiology and biotechnology , ecology , mutant
In the quest for plant regulatory sequences capable of driving nematode-triggered effector gene expression in feeding structures, we show that promoter tagging is a valuable tool. A large collection of transgenic Arabidopsis plants was generated. They were transformed with a beta-glucuronidase gene functioning as a promoter tag. Three T-DNA constructs, pGV1047, p delta gusBin19, and pMOG553, were used. Early responses to nematode invasion were of primary interest. Six lines exhibiting beta-glucuronidase activity in syncytia induced by the beet cyst nematode were studied. Reporter gene activation was also identified in galls induced by root knot and ectoparasitic nematodes. Time-course studies revealed that all six tags were differentially activated during the development of the feeding structure. T-DNA-flanking regions responsible for the observed responses after nematode infection were isolated and characterized for promoter activity.
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